CATALASE AND HYDROGEN PEROXIDE EXPERIMENT COURSEWORK

This is because at higher concentrations, there are more substrate molecules than in lower concentrations. If you want more detailed biology information then I’d recommend the book ‘Advanced Biology’ by M. Placed 5 test tubes in each test tube rack. If my replicates had not been so close I would have had to change the tube. In addition, the rate of decomposition did not stop increasing and did not stay constant with increasing catalase concentration.

Ignore the last question What board gave the grade? This means that the reaction a first-order reaction, so the rate is proportional to the concentration. This is used to collect data on traffic to articles and other pages on our site. In this case, I will keep the volume of hydrogen peroxide the same for using each time. Set up your apparatus. This is feature allows you to search the site.

catalase and hydrogen peroxide experiment coursework

Good luck with your coursework. If my replicates had not been so close I would have had to change the tube. Skip to main content. Potato catalase pH – answers. Catalase dramatically reduces the activation energy needed for the reaction. This meant that because the enzyme concentration catalase in yeast was reduced, there were pefoxide collisions between enzyme and substrate molecules, so the rate of enzyme-substrate formations was reduced.

All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available.

Effect of Substrate Concentration on the Rate of Activity of Catalase | Owlcation

The substrate molecule is held within the active site by bonds that temporarily form between the R groups of the amino acids of the active site by bonds and all groups on the substrate molecules.

This is shown when oxygen stops being produced and the same results are recorded five times. The table below shows what amounts of Hydrogen Peroxide and water are needed to make the solutions. However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must hydrofen that there was a similar number of substrate molecules in each concentration.

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If the catalase was used up then another potato would have to be crushed and this could produce catalase of a totally different concentration which would lead to inaccuracies in the experiment making this an unfair test.

size gcse coursework biology potato catalase hydrogen peroxide

Free catalase Essays and Papers – helpme. I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate. I predict that as the peroxkde concentration increases, the rate of reaction will go up at a directly proportional rate until the solution becomes saturated with the substrate hydrogen peroxide.

catalase and hydrogen peroxide experiment coursework

When this saturation point is reached, then adding extra substrate will make no difference. At a temperature below the optimum, the molecules have less kinetic energy, so the rate of collisions between enzyme and substrate molecules is low, therefore less enzyme-substrate complexes are formed. In Store Pickup Available. Therefore if the concentration of enzyme is increased, the number of collision with the substrate molecule and hence the rate of reaction will increase.

These might cause unequal distribution of catalase which will directly impact the results. Because he adds water to the yeast not to the peroxide to change the concentration. Catalase is an enzyme found in food such as potato and liver.

This will ensure that since the temperature is below the optimum, the reaction will be slower and therefore enable me to collect oxygen at a measurable rate. As globular protein, enzymes have a specific three-dimensional shape which is determined by their sequence htdrogen amino acids. The rate of reaction can be measured by measuring the volume of oxygen produced in experimebt period of time.

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Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide

I predict that the higher the concentration of celery extract I use, the fast the rate of breaking down hydrogen peroxide I will get. This heat energy is transferred to coursswork environment. Use a pipette rather than a conical flask or a measuring cylinder because pipettes are very accurate for measuring volumes.

Considering the whole experiment, 5. The method which I used to do this can be seen below.

catalase and hydrogen peroxide experiment coursework

To ensure this is a hgdrogen test all the variables except for the concentration of Hydrogen Peroxide must be kept the same for all the experiments. The hydrogen peroxide is inserted into a 5cm 3 beaker and then tipped over to ‘spill’ the contents and start the reaction. In this case, I will use hydrogen peroxide as the substrate.

This is because fewer overall collisions took place, and so catalawe reduced number of collisions reached the activation energy. The dependent variable the one I intend to measure is the volume of gas produced in each reaction. The Coursework on Rate Of We extract catalase from grinded potatoes and add increasing amount of catalase to hydrogen peroxide in separate test tubes.

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